Doe J E, Hicks R, Milburn G M
Int Arch Allergy Appl Immunol. 1982;68(3):275-9. doi: 10.1159/000233111.
Guinea pigs were exposed by head only inhalation for 5 h to concentrations of approximately 1 ppm of tolylene diisocyanate (TDI) and 14 days later pinnal application of TDI on 3 consecutive days was followed by a dermal application of TDI after a further 7 days. The degree of contact sensitivity, measured 24 h later, showed that prior inhalation inhibited the skin reaction to TDI but it did not change the skin reaction to chloro-dinitrobenzene (DNCB) in guinea pigs sensitised and challenged similarly with DNCB. The inhibition lasted up to 9 weeks. However, it did not occur in guinea pigs after intranasal instillation (instead of inhalation) and only occurred after oral dosage with TDI when the dose was relatively high. Treatment with cyclophosphamide before TDI inhalation reversed the inhibition of contact sensitivity, suggesting an involvement of suppressor cells.
将豚鼠仅头部吸入约1 ppm的甲苯二异氰酸酯（TDI）5小时，14天后连续3天耳廓涂抹TDI，再过7天后进行TDI的皮肤涂抹。24小时后测量的接触敏感性程度表明，预先吸入会抑制豚鼠对TDI的皮肤反应，但对用二硝基氯苯（DNCB）进行类似致敏和激发的豚鼠，它不会改变其对DNCB的皮肤反应。这种抑制持续长达9周。然而，在豚鼠经鼻滴注（而非吸入）TDI后未出现这种抑制，且仅在口服相对高剂量TDI时才会出现。在吸入TDI前用环磷酰胺治疗可逆转接触敏感性的抑制，提示有抑制细胞参与。
