Sera N, Tokiwa H, Hirohata T
Department of Health Science, Fukuoka Environmental Research Center, Japan.
Toxicol Lett. 1990 Feb;50(2-3):289-98. doi: 10.1016/0378-4274(90)90022-e.
Samples of cigarette smoke condensates (CSCs) treated with nitric acid or Chinese cabbage pickles having a high nitrate content strongly mutated Salmonella typhimurium strain TA98; the benzene/ethanol extract after these treatments induced 1800 and 820 revertants, respectively, per mg of extract for strain TA98 in the absence of S9 mix. The major mutagens in these materials were found to be 1-nitropyrene and 1,3-dinitropyrene on the basis of the result of gas chromatography/mass spectrometry and of fluorescence spectra of the samples. The quantity of 1-nitropyrene was 14.5 ng per cigarette for the CSCs treated with nitric acid, and for 11.2 ng for those treated with Chinese cabbage pickles. Similarly, 1,3-dinitropyrene was detected in the CSCs treated with nitric acid or Chinese cabbage pickles at concentrations of 0.38 and approximately 0.1 ng, respectively, per cigarette.
用硝酸处理过的香烟烟雾冷凝物（CSCs）样本或含有高硝酸盐含量的大白菜泡菜能强烈诱变鼠伤寒沙门氏菌TA98菌株；在不存在S9混合物的情况下，这些处理后的苯/乙醇提取物每毫克提取物分别诱导TA98菌株产生1800个和820个回复突变体。根据气相色谱/质谱结果和样品的荧光光谱，发现这些物质中的主要诱变剂是1-硝基芘和1,3-二硝基芘。用硝酸处理的CSCs中1-硝基芘的含量为每支香烟14.5纳克，用大白菜泡菜处理的为11.2纳克。同样，在用硝酸或大白菜泡菜处理的CSCs中分别检测到1,3-二硝基芘，浓度分别为每支香烟0.38纳克和约0.1纳克。
