Ohe T, Nukaya H
Department of Food and Nutrition Science, Kyoto Women's University, Higashiyama-ku, Japan.
Sci Total Environ. 1996 Mar 1;181(1):7-12. doi: 10.1016/0048-9697(95)04952-5.
The identification of mutagenic 1-nitropyrene, one of the direct-acting nitroarenes, in a source of municipal drinking water is described. A diethyl ether extract recovered from river water by the XAD-2 resin column method was separated into neutral, acid and basic fractions. The neutral fraction accounted for 52 and 36%, respectively, of the genotoxicity of the extract in the absence and the presence of the metabolic activation system, for strain NM2009, which is sensitive to nitroarenes and/or aminoarenes. When separated by silica gel column chromatography, the benzene fraction of the neutral fraction, showed the highest genotoxic activity. The genotoxicity of the benzene fraction accounted for 80 and 60%, respectively, of the neutral fraction, in the absence and the presence of S9 mix. These results show that mutagenic nitroarenes might be contained in the benzene fraction of the neutral one. The benzene fraction was further subjected to HPLC and fractionated. A 1-nitropyrene-corresponding fraction was collected and subjected to capillary gas chromatography-mass spectrometry (GC-MS). The mass spectrum showed molecular and fragment ion peaks of 1-nitropyrene: 247, 217, 201, 189. Moreover, the concentration of 1-nitropyrene in municipal river water was 1 ng/l, accounting for only 1% of the total genotoxicity.
本文描述了在城市饮用水源中鉴定出诱变剂1-硝基芘，它是一种直接作用的硝基芳烃。通过XAD - 2树脂柱法从河水中回收的二乙醚提取物被分离为中性、酸性和碱性馏分。对于对硝基芳烃和/或氨基芳烃敏感的菌株NM2009，在不存在和存在代谢活化系统的情况下，中性馏分分别占提取物遗传毒性的52%和36%。当通过硅胶柱色谱分离时，中性馏分的苯馏分显示出最高的遗传毒性活性。在不存在和存在S9混合液的情况下，苯馏分的遗传毒性分别占中性馏分的80%和60%。这些结果表明，诱变硝基芳烃可能存在于中性馏分的苯馏分中。对苯馏分进一步进行高效液相色谱（HPLC）分离和分级。收集到一个与1-硝基芘对应的馏分，并进行毛细管气相色谱 - 质谱联用（GC - MS）分析。质谱图显示出1-硝基芘的分子离子峰和碎片离子峰：247、217、201、189。此外，城市河水中1-硝基芘的浓度为1 ng/L，仅占总遗传毒性的1%。
