在致病性酵母新生隐球菌中高效实施 RNA 干扰。
Efficient implementation of RNA interference in the pathogenic yeast Cryptococcus neoformans.
机构信息
Department of Molecular Microbiology, Washington University School of Medicine, Campus Box 8230, 660 South Euclid Avenue, St. Louis, MO 63110, USA.
出版信息
J Microbiol Methods. 2011 Aug;86(2):156-9. doi: 10.1016/j.mimet.2011.04.014. Epub 2011 Apr 29.
Abstract
An improved method has been developed for RNA interference in Cryptococcus neoformans, using opposing promoters to facilitate cloning and RNA interference targeting URA5 to allow selection of cells in which silencing is most effective. These advances significantly reduce the variability of silencing and the effort required for interference plasmid construction.
摘要
已开发出一种改良的新型隐球菌 RNA 干扰方法，使用相反的启动子来促进 URA5 的克隆和 RNA 干扰靶向，从而可以选择沉默最有效的细胞。这些进展显著降低了沉默的可变性和干扰质粒构建所需的工作量。
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