Rosolen A, Frascella E, Toretsky J, Neckers L
NCI,BETHESDA,MD.
Int J Oncol. 1995 Jan;6(1):175-9. doi: 10.3892/ijo.6.1.175.
The neuroepithelioma cell line CHP100 expresses low but detectable amounts of N-myc protein together with large amounts of c-myc protein. We have recently demonstrated that antisense inhibition of N-myc expression in CHP100 cells leads to decreased in vitro growth and alterations in cellular morphology without affecting tumorigenicity in nude mice. In this study we report the construction of an episomally replicating vector designed to generate RNA antisense to part of the human c-myc gene. Such a Vector is able to inhibit c-myc expression in cell lines carrying multiple copies of the gene. Inhibition of c-myc expression leads to a decrease of in vitro growth and cloning efficiency and in vivo tumorigenicity of CHP100 cells. Our findings suggest that N-myc and c-myc subserve different functions in regulating the biology of CHP100 cells.
神经上皮瘤细胞系CHP100表达少量但可检测到的N - myc蛋白，同时表达大量的c - myc蛋白。我们最近证明，对CHP100细胞中N - myc表达进行反义抑制会导致体外生长减少和细胞形态改变，而不影响裸鼠的致瘤性。在本研究中，我们报告了一种游离复制载体的构建，该载体旨在产生针对人c - myc基因部分区域的RNA反义序列。这样的载体能够抑制携带该基因多个拷贝的细胞系中的c - myc表达。抑制c - myc表达会导致CHP100细胞的体外生长、克隆效率以及体内致瘤性降低。我们的研究结果表明，N - myc和c - myc在调节CHP100细胞生物学特性方面发挥不同的功能。
