Rosolen A, Whitesell L, Ikegaki N, Kennett R H, Neckers L M
Tumor Cell Biology Section, National Cancer Institute, NIH, Bethesda, Maryland 20892.
Cancer Res. 1990 Oct 1;50(19):6316-22.
The N-myc gene is transiently expressed during normal embryonic development and abnormally expressed in several tumors of neuroendocrine origin. Little is known of the function of the N-myc gene product in either normal or neoplastic tissue. We utilized synthetic antisense oligodeoxynucleotides to specifically inhibit N-myc gene expression in the neuroepithelioma cell line CHP100. These cells contain single copy N-myc alleles but overexpress c-myc. N-myc antisense oligomer treatment was found to be growth inhibitory without affecting levels of c-myc protein. N-myc antisense oligomer-treated cells also lost the characteristic cellular heterogeneity displayed by CHP100 in vitro.
N-myc基因在正常胚胎发育过程中短暂表达，在几种神经内分泌起源的肿瘤中异常表达。关于N-myc基因产物在正常组织或肿瘤组织中的功能，人们所知甚少。我们利用合成的反义寡脱氧核苷酸特异性抑制神经上皮瘤细胞系CHP100中的N-myc基因表达。这些细胞含有单拷贝N-myc等位基因，但c-myc过度表达。发现N-myc反义寡聚物处理具有生长抑制作用，而不影响c-myc蛋白水平。经N-myc反义寡聚物处理的细胞在体外也失去了CHP100所显示的特征性细胞异质性。
