Wrightperkins S, Daniel M, Walker C
CLATTERBRIDGE HOSP,JK DOUGLAS CANC RES LABS,CLATTERBRIDGE CANC RES TRUST,WIRRAL L63 4JY,MERSEYSIDE,ENGLAND.
Int J Oncol. 1995 Mar;6(3):617-23. doi: 10.3892/ijo.6.3.617.
1 PT and 1 PT variant A clonal cell lines, both derived from the same neuroendocrine positive, undifferentiated large cell lung carcinoma, showed differences in their morphology, DNA content, presence of desmosomes and expression of NCAM and desmoplakin. Colonies of cells which morphologically resembled 1 PT variant A cells arose in cultures of the 1 PT clonal line following prolonged cultivation. Such transitions in cloned cells of NSCLC origin have not previously been reported. This transition was associated with increased expression of desmoplakin and downregulation of NCAM. A converse transition of 1 PT variant A cells to 1 PT-like cells could not be demonstrated.
1个PT细胞系和1个PT变异A细胞系均源自同一神经内分泌阳性的未分化大细胞肺癌，它们在形态、DNA含量、桥粒的存在以及神经细胞黏附分子（NCAM）和桥粒斑蛋白的表达方面存在差异。在1个PT细胞系的培养物中，经过长时间培养后出现了形态上类似于1个PT变异A细胞的细胞集落。此前尚未报道过非小细胞肺癌（NSCLC）来源的克隆细胞发生这种转变。这种转变与桥粒斑蛋白表达增加和NCAM下调有关。未能证实1个PT变异A细胞向1个PT样细胞的反向转变。
