Jiang W G, Singhrao S K, Hiscox S, Hallett M B, Bryce R P, Horrobin D F, Puntis M C, Mansel R E
Metastasis Research Group, University Department of Surgery, University of Wales College of Medicine, Cardiff, UK.
Clin Exp Metastasis. 1997 Nov;15(6):593-602. doi: 10.1023/a:1018435229087.
Desmosomes are key structures in cell-cell adhesion. In this study we examined the effect of n-6 essential fatty acids on the expression of desmoglein (Dsg), desmosomal cadherin and the formation of desmosomes in E-cadherin negative human breast, colon and lung cancer cells and melanoma cells. Electron microscopy revealed that cells cultured with gamma linolenic acid (GLA) showed increased cell-cell adhesion together with an increase in the formation of desmoglein-containing desmosomes. Western blotting studies of cellular proteins demonstrated that, following culture with fatty acids, Dsg expression was modified, with the greatest increase seen after GLA treatment. Other fatty acids increased Dsg expression, but to a lesser extent. It is concluded that GLA regulates desmosome-mediated cell-cell adhesion in human cancer cells, particularly in cells without E-cadherin.
桥粒是细胞间黏附的关键结构。在本研究中，我们检测了n-6必需脂肪酸对桥粒芯糖蛋白（Dsg）、桥粒钙黏蛋白的表达以及E-钙黏蛋白阴性的人乳腺癌、结肠癌、肺癌细胞和黑色素瘤细胞中桥粒形成的影响。电子显微镜显示，用γ-亚麻酸（GLA）培养的细胞表现出细胞间黏附增加，同时含桥粒芯糖蛋白的桥粒形成也增加。细胞蛋白质的蛋白质印迹研究表明，在用脂肪酸培养后，Dsg表达发生改变，GLA处理后增加最为显著。其他脂肪酸也增加了Dsg表达，但程度较小。得出的结论是，GLA调节人癌细胞中桥粒介导的细胞间黏附，尤其是在没有E-钙黏蛋白的细胞中。
