Université de Bordeaux, CNRS UMR 5248 CBMN, 2 Rue Robert Escarpit, 33604 Pessac, France.
Anal Bioanal Chem. 2011 Aug;401(3):795-801. doi: 10.1007/s00216-011-5069-1. Epub 2011 May 10.
Fourier-transform infrared (FTIR) imaging has been used to investigate brain tumor angiogenesis using a mice solid tumor model and bare-gold (∅ 25 nm) or BaSO(4) (∅ 500 nm) nanoparticles (NP) injected into blood vasculature. FTIR images of 20-μm-thick tissue sections were used for chemical histology of healthy and tumor areas. Distribution of BaSO(4)-NP (using the 1,218-1,159 cm(-1) spectral interval) revealed clearly all details of blood vasculature with morphological abnormalities of tumor capillaries, while Au-NP (using the 1,046-1,002 cm(-1) spectral interval) revealed also diffusion properties of leaky blood vessels. Diffusion of Au-NP out of vascular space reached 64 ± 29 μm, showing the fenestration of "leaky" tumor blood vessels, which should allow small NP (<100 nm, as for Au-NP) to diffuse almost freely, while large NP should not (as for BaSO(4)-NP in this study). Therefore, we propose to develop FTIR imaging as a convenient tool for functional molecular histology imaging of brain tumor vasculature, both for identifying blood capillaries and for determining the extravascular diffusion space offered by vessel fenestration.
傅里叶变换红外（FTIR）成像已被用于使用小鼠实体瘤模型和裸金（∅25nm）或 BaSO4（∅500nm）纳米颗粒（NP）注入血管系统来研究脑肿瘤血管生成。使用 20μm 厚的组织切片的 FTIR 图像用于健康和肿瘤区域的化学组织学。BaSO4-NP（使用 1218-1159cm-1光谱间隔）的分布清楚地揭示了所有具有肿瘤毛细血管形态异常的血管细节，而 Au-NP（使用 1046-1002cm-1光谱间隔）也揭示了漏血管的扩散特性。Au-NP 从血管空间扩散到 64±29μm，显示出“渗漏”肿瘤血管的窗孔，这应该允许小 NP（<100nm，如 Au-NP）几乎自由扩散，而大 NP 不应（如本研究中的 BaSO4-NP）。因此，我们建议将 FTIR 成像开发为脑肿瘤血管功能分子组织学成像的便捷工具，既可以识别血毛细血管，也可以确定由血管窗孔提供的血管外扩散空间。
