Valorisation of Plant Production Chains, Wageningen University, Wageningen, the Netherlands.
Appl Microbiol Biotechnol. 2011 Jun;90(6):2027-36. doi: 10.1007/s00253-011-3312-5. Epub 2011 May 10.
We investigated the use of bacterial cells isolated from paddy crab for the extraction of oil from Jatropha seed kernels in aqueous media while simultaneously preserving the protein structures of this protein-rich endosperm. A bacterial strain-which was marked as MB4 and identified by means of 16S rDNA sequencing and physiological characterization as either Bacillus pumilus or Bacillus altitudinis-enhanced the extraction yield of Jatropha oil. The incubation of an MB4 starter culture with preheated kernel slurry in aqueous media with the initial pH of 5.5 at 37 °C for 6 h liberated 73% w/w of the Jatropha oil. Since MB4 produces xylanases, it is suggested that strain MB4 facilitates oil liberation via degradation of hemicelluloses which form the oil-containing cell wall structure of the kernel. After MB4 assisted oil extraction, SDS-PAGE analysis showed that the majority of Jatropha proteins were preserved in the solid phase of the extraction residues. The advantages offered by this process are: protein in the residue can be further processed for other applications, no purified enzyme preparation is needed, and the resulting oil can be used for biodiesel production.
我们研究了从稻田蟹中分离出的细菌细胞在水介质中从麻疯树种子仁中提取油的用途，同时保持了富含蛋白质的胚乳的蛋白质结构。一种细菌菌株（被标记为 MB4 号，并通过 16S rDNA 测序和生理特征鉴定为短小芽孢杆菌或地衣芽孢杆菌）提高了麻疯树油的提取产量。将 MB4 号起始培养物与预热的仁浆在初始 pH 值为 5.5 的水介质中于 37°C 孵育 6 小时，可释放 73%w/w 的麻疯树油。由于 MB4 号产生木聚糖酶，因此推测该菌株通过降解形成仁细胞壁结构的半纤维素来促进油的释放。在 MB4 号辅助油提取之后，SDS-PAGE 分析表明，大多数麻疯树蛋白保留在提取残渣的固相中。该工艺的优点是：残渣中的蛋白质可进一步用于其他应用，无需纯化酶制剂，并且得到的油可用于生物柴油生产。
