Faculty of Life and Social Sciences, Swinburne University of Technology, PO Box 218, Hawthorn, Melbourne, VIC 3122, Australia.
Appl Microbiol Biotechnol. 2011 Aug;91(4):1149-57. doi: 10.1007/s00253-011-3195-5. Epub 2011 May 10.
The physicochemical and bactericidal properties of thin silver films have been analysed. Silver films of 3 and 150 nm thicknesses were fabricated using a magnetron sputtering thin-film deposition system. X-ray photoelectron and energy dispersive X-ray spectroscopy and atomic force microscopy analyses confirmed that the resulting surfaces were homogeneous, and that silver was the most abundant element present on both surfaces, being 45 and 53 at.% on the 3- and 150-nm films, respectively. Inductively coupled plasma time of flight mass spectroscopy (ICP-TOF-MS) was used to measure the concentration of silver ions released from these films. Concentrations of 0.9 and 5.2 ppb were detected for the 3- and 150-nm films, respectively. The surface wettability of the films remained nearly identical for both film thicknesses, displaying a static water contact angle of 95°, while the surface free energy of the 150-nm film was found to be slightly greater than that of the 3-nm film, being 28.8 and 23.9 mN m(-1), respectively. The two silver film thicknesses exhibited statistically significant differences in surface topographic profiles on the nanoscopic scale, with R (a), R (q) and R (max) values of 1.4, 1.8 and 15.4 nm for the 3-nm film and 0.8, 1.2 and 10.7 nm for the 150-nm film over a 5 × 5 μm scanning area. Confocal scanning laser microscopy and scanning electron microscopy revealed that the bactericidal activity of the 3-nm silver film was not significant, whereas the nanoscopically smoother 150-nm silver film exhibited appreciable bactericidal activity towards Pseudomonas aeruginosa ATCC 9027 cells and Staphylococcus aureus CIP 65.8 cells, obtaining up to 75% and 27% sterilisation effect, respectively.
已经分析了薄银膜的物理化学和杀菌性能。使用磁控溅射薄膜沉积系统制备了 3nm 和 150nm 厚的银膜。X 射线光电子能谱和能量色散 X 射线能谱以及原子力显微镜分析证实，所得表面是均匀的，并且两种表面上最丰富的元素都是银，分别为 3nm 和 150nm 薄膜上的 45%和 53%。电感耦合等离子体质谱（ICP-TOF-MS）用于测量从这些薄膜中释放的银离子的浓度。检测到 3nm 和 150nm 薄膜的浓度分别为 0.9ppb 和 5.2ppb。两种薄膜厚度的表面润湿性几乎相同，均显示静态水接触角为 95°，而 150nm 薄膜的表面自由能略大于 3nm 薄膜，分别为 28.8 和 23.9mN m(-1)。两种银膜厚度在纳米尺度的表面形貌轮廓上表现出统计学上显著的差异，3nm 薄膜的 R(a)、R(q)和 R(max)值分别为 1.4nm、1.8nm 和 15.4nm，150nm 薄膜的 R(a)、R(q)和 R(max)值分别为 0.8nm、1.2nm 和 10.7nm，扫描面积为 5×5μm。共焦扫描激光显微镜和扫描电子显微镜显示，3nm 银膜的杀菌活性不显著，而纳米级更光滑的 150nm 银膜对铜绿假单胞菌 ATCC 9027 细胞和金黄色葡萄球菌 CIP 65.8 细胞具有明显的杀菌活性，分别获得了高达 75%和 27%的杀菌效果。
