School of Chemistry, University of New South Wales, Sydney, NSW, Australia.
Biofouling. 2010 Nov;26(8):913-21. doi: 10.1080/08927014.2010.531463.
Bacterial infection of biomedical devices is still a major barrier to their use. This is compounded by increasing antibiotic resistance. Here, the specific covalent attachment of a series of dihydropyrrol-2-one (DHP), analogues of bacterial quorum sensing inhibitors, to surfaces via a Michael-type addition reaction is described. Differences in efficiency of attachment related to the substituent groups were found by X-ray photoelectron spectroscopy. The physical characteristics of the surfaces were further explored by atomic force microscopy and contact angle measurements. The ability of these coatings to prevent the formation of a biofilm by Pseudomonas aeruginosa and Staphylococcus aureus was examined using confocal laser scanning microscopy and image analysis. The DHP-treated surfaces showed significant reductions in bacterial adhesion without increased killing for both strains of bacteria (p < 0.001). 5-Methylene-1-(prop-2-enoyl)-4-phenyl-dihydropyrrol-2-one was identified as having broad spectrum activity and consequently represents an excellent candidate for the development of novel surfaces for the prevention of biomedical device infections.
生物医学设备的细菌感染仍然是其应用的主要障碍。这因抗生素耐药性的增加而更加复杂。在这里，通过迈克尔型加成反应，将一系列二氢吡咯-2-酮（DHP），细菌群体感应抑制剂的类似物，通过特定的共价键连接到表面上。通过 X 射线光电子能谱发现了与取代基有关的附着效率的差异。通过原子力显微镜和接触角测量进一步探索了表面的物理特性。使用共聚焦激光扫描显微镜和图像分析检查了这些涂层防止铜绿假单胞菌和金黄色葡萄球菌形成生物膜的能力。用 DHP 处理的表面显示出细菌粘附的显着减少，而对两种细菌的杀伤均没有增加（p <0.001）。5-亚甲基-1-（丙烯酰基）-4-苯基-二氢吡咯-2-酮被鉴定为具有广谱活性，因此是开发用于预防生物医学设备感染的新型表面的理想候选物。
