原子力显微镜研究与细菌相关的分子间力和键。

Atomic force microscopy to study intermolecular forces and bonds associated with bacteria.

机构信息

Ohio State University, Columbus, OH, 43210, USA.

出版信息

Adv Exp Med Biol. 2011;715:285-99. doi: 10.1007/978-94-007-0940-9_18.

Abstract

Atomic force microscopy (AFM) operates on a very different principle than other forms of microscopy, such as optical microscopy or electron microscopy. The key component of an AFM is a cantilever that bends in response to forces that it experiences as it touches another surface. Forces as small as a few picoNewtons can be detected and probed with AFM. AFM has become very useful in biological sciences because it can be used on living cells that are immersed in water. AFM is particularly useful when the cantilever is modified with chemical groups (e.g. amine or carboxylic groups), small beads (e.g. glass or latex), or even a bacterium. This chapter describes how AFM can be used to measure forces and bonds between a bacterium and another surface. This paper also provides an example of the use of AFM on Staphylococcus aureus, a Gram-positive bacterium that is often associated with biofilms in humans.

摘要

原子力显微镜（AFM）的工作原理与其他形式的显微镜（如光学显微镜或电子显微镜）有很大的不同。AFM 的关键组件是一个悬臂梁，当它接触到另一个表面时，它会根据所经历的力而弯曲。AFM 可以检测到小至几个皮牛顿的力，并进行探测。AFM 在生物科学中非常有用，因为它可以用于浸没在水中的活细胞。当悬臂梁用化学基团（如胺基或羧基）、小珠子（如玻璃或乳胶），甚至是细菌进行修饰时，AFM 尤其有用。本章介绍了如何使用 AFM 测量细菌与另一个表面之间的力和键。本文还提供了一个使用 AFM 研究金黄色葡萄球菌（一种革兰氏阳性菌，常与人类生物膜有关）的例子。