Molecular and Cellular Toxicology Section, Laboratory of Molecular Immunology, National Heart, Lung, and Blood Institute, National Institutes of Health, Department of Health and Human Services, Bethesda, Maryland 20892-1760, United States.
Chem Res Toxicol. 2011 Jun 20;24(6):794-6. doi: 10.1021/tx200143x. Epub 2011 May 24.
C57BL/6 mice are widely used in biomedical research for the background of genetically engineered mice (GEM) and wild-type controls with the belief that the genetic background of GEM and control mice differ significantly by only one or more altered genes. This principle, however, does have limitations due in part to the existence of multiple substrains of C57BL/6 mice that should not be used interchangeably as they can differ both genetically and phenotypically. We show here that these mispairings do occur frequently and can lead to inaccurate and conflicting findings.
C57BL/6 小鼠广泛应用于生物医学研究，其背景是基因工程小鼠（GEM）和野生型对照小鼠，人们认为 GEM 和对照小鼠的遗传背景仅因一个或多个改变的基因而有显著差异。然而，这一原则确实存在局限性，部分原因是 C57BL/6 小鼠存在多个亚系，这些亚系不应互换使用，因为它们在遗传和表型上都可能存在差异。我们在这里表明，这些错配经常发生，并可能导致不准确和相互矛盾的发现。
