Liu Ming, Wen Yangping, Xu Jingkun, He Haohua, Li Dong, Yue Ruirui, Liu Guodong
Jiangxi Key Laboratory of Organic Chemistry, Jiangxi Science and Technology Normal University, Nanchang, China.
Anal Sci. 2011;27(5):477. doi: 10.2116/analsci.27.477.
An amperometric L-ascorbic acid (AA) biosensor fabricated by immobilizing ascorbate oxidase (AO) in poly(3,4-ethylenedioxythiophene) (PEDOT) and multi-walled carbon nanotubes (MWCNTs) composite films was reported for the first time. The entrapment of AO in PEDOT/MWCNTs composite films was performed during an electrochemical polymerization process. The influence of various experimental conditions was examined for determining the optimum analytical performance. The response of the biosensor towards AA under the optimized conditions is linear from 0.05 to 20 mM with a detection limit of 15 µM (S/N = 3). The biosensor shows a response time of 20 s and a sensitivity of 23.95 mA M(-1) cm(-2). The apparent Michaelis-Menten constant (K(m)) and apparent activation energy (E(a)) are 19.5 mM and 21 kJ mol(-1), respectively. Moreover, the biosensor exhibits good anti-interferent ability, good reproducibility and remarkable storage stability.
首次报道了一种通过将抗坏血酸氧化酶（AO）固定在聚（3,4-乙撑二氧噻吩）（PEDOT）和多壁碳纳米管（MWCNT）复合膜中制备的电流型L-抗坏血酸（AA）生物传感器。在电化学聚合过程中进行AO在PEDOT/MWCNT复合膜中的包埋。研究了各种实验条件的影响以确定最佳分析性能。在优化条件下，该生物传感器对AA的响应在0.05至20 mM范围内呈线性，检测限为15 μM（S/N = 3）。该生物传感器的响应时间为20 s，灵敏度为23.95 mA M(-1) cm(-2)。表观米氏常数（K(m)）和表观活化能（E(a)）分别为19.5 mM和21 kJ mol(-1)。此外，该生物传感器具有良好的抗干扰能力、良好的重现性和显著的储存稳定性。
