Çetin Merih Zeynep, Camurlu Pinar
Akdeniz University, Department of Chemistry 07058 Antalya Turkey
RSC Adv. 2018 May 29;8(35):19724-19731. doi: 10.1039/c8ra01385c. eCollection 2018 May 25.
Here we present a simple, low cost approach for the production of PEDOT nanofiber biosensors using simple techniques. Firstly, nanofibers of PEDOT were produced by the chemical vapor polymerization of EDOT on FeCl containing electrospun PAN nanofiber mats. The nanofibers were characterized by SEM, FTIR, CV and conductivity studies, which indicated the formation of homogeneous, porous, electroactive PEDOT nanofibers. The fabrication of biosensors was achieved through the loading of various amounts of GOx on the nanofibers. To uncover their capability, the biosensors were operated under both hydrogen peroxide production and oxygen consumption conditions. For each biosensor current response glucose concentration calibration curves were plotted. The sensitivity, linear range, LOD, and values of the biosensors were determined and the stabilities of all the sensors were investigated. The biosensor operating at 0.6 V revealed a lower LOD with a wider linear range, higher stability, good sensitivity and selectivity. For example, the PEDOT-NFs/GOx-3 nanofiber biosensor showed good sensitivity (74.22 μA mM cm) and LOD (2.9 μM) with a response time of 2-3 s without any interference effects. The PEDOT-NFs/GOx-2 biosensor operating at -0.6 V exhibited extreme sensitivity of 272.58 μA mM cm. Our studies have shown that having good sensitivity, LOD and stability makes these interference-free, easy to construct sensors viable candidates for commercialization.
在此，我们展示了一种使用简单技术生产聚（3,4-乙撑二氧噻吩）（PEDOT）纳米纤维生物传感器的简单、低成本方法。首先，通过在含氯化铁的静电纺丝聚丙烯腈（PAN）纳米纤维毡上对3,4-乙撑二氧噻吩（EDOT）进行化学气相聚合来制备PEDOT纳米纤维。通过扫描电子显微镜（SEM）、傅里叶变换红外光谱（FTIR）、循环伏安法（CV）和电导率研究对纳米纤维进行了表征，结果表明形成了均匀、多孔、具有电活性的PEDOT纳米纤维。通过在纳米纤维上负载不同量的葡萄糖氧化酶（GOx）来实现生物传感器的制造。为了揭示其性能，在过氧化氢产生和氧气消耗条件下对生物传感器进行操作。针对每个生物传感器绘制了电流响应-葡萄糖浓度校准曲线。测定了生物传感器的灵敏度、线性范围、检测限（LOD）以及其他参数，并研究了所有传感器的稳定性。在0.6 V下运行的生物传感器显示出较低的检测限、较宽的线性范围、更高的稳定性、良好的灵敏度和选择性。例如，PEDOT-NFs/GOx-3纳米纤维生物传感器表现出良好的灵敏度（74.22 μA mM⁻¹ cm⁻²）和检测限（2.9 μM），响应时间为2 - 3 s，且无任何干扰效应。在 - 0.6 V下运行的PEDOT-NFs/GOx-2生物传感器表现出高达272.58 μA mM⁻¹ cm⁻²的极高灵敏度。我们的研究表明，这些具有良好灵敏度、检测限和稳定性且无干扰、易于构建的传感器是商业化的可行候选者。
