Onal A, Oğüş A, Kisakürek D
Department of Science, Middle-East Technical University, Ankara, Turkey.
J Biochem Biophys Methods. 1990 Jan;20(2):137-42. doi: 10.1016/0165-022x(90)90072-k.
A new method for the production of ascorbate free radicals is established. The radical is produced from ascorbate in deionized water by applying constant potential electrolysis under a nitrogen atmosphere. Prior to electrolysis, a cyclic voltammogram (CV) of the ascorbic acid was obtained. Electrolysis potentials were selected as the oxidation peak potential of the ascorbic acid obtained by CV. The detection of the radical was done by electron spin resonance (esr) and uv spectroscopies.
建立了一种生产抗坏血酸自由基的新方法。该自由基是在氮气气氛下通过恒电位电解，由去离子水中的抗坏血酸产生的。在电解之前，获得了抗坏血酸的循环伏安图（CV）。电解电位选择为通过CV获得的抗坏血酸的氧化峰电位。通过电子自旋共振（ESR）和紫外光谱对自由基进行检测。
