Department of Otolaryngology-Head & Neck Surgery, Chang Gung Memorial Hospital at Lin-Kou, Taiwan, Republic of China.
Head Neck. 2011 Jun;33(6):886-97. doi: 10.1002/hed.21557. Epub 2010 Nov 29.
The purpose of this study was to examine cytokine profiles of peripheral blood for nasopharyngeal carcinoma (NPC).
A total of 132 patients with untreated NPC, 169 healthy individuals, and 32 patients with chronic rhinosinusitis (CRS) were enrolled in this study. The plasma levels of 13 cytokines were measured by the multiplexed immunobead-based method.
The levels of interleukin (IL)-6, IL-8, interferon-inducible protein 10 (IP-10), tumor necrosis factor (TNF)-α, vascular endothelial growth factor (VEGF), and macrophage inflammatory protein (MIP)-3α were significantly elevated in patients with NPC; but the stem cell factor (SCF) levels were significantly lower. The levels of IL-6, IL-8, IP-10, and MIP-3α were significantly elevated in patients with advanced stages disease and correlated with Epstein-Barr virus (EBV) DNA. A 2-marker panel consisting of MIP-3α and SCF increased the screening efficacy of EBV-specific viral capsid antigen (VCA) IgA or EBV DNA, although the addition of MIP-3α and SCF to the combined set of currently available markers (EBV VCA IgA and EBV DNA) did not improve the accuracy of the diagnostic panel. The patients with NPC with higher levels of IL-8, VEGF, MIP-3α, and EBV DNA had worse prognoses for overall survival (p = .008, .035, .005, and .007, respectively).
Simultaneous, large-scale measurement of multiple cytokines may improve NPC detection and prognostic prediction.
本研究旨在研究鼻咽癌（NPC）患者外周血的细胞因子谱。
本研究共纳入 132 例未经治疗的 NPC 患者、169 名健康对照者和 32 例慢性鼻-鼻窦炎（CRS）患者。采用基于多重免疫珠的方法检测血浆中 13 种细胞因子的水平。
NPC 患者的白细胞介素（IL）-6、IL-8、干扰素诱导蛋白 10（IP-10）、肿瘤坏死因子（TNF）-α、血管内皮生长因子（VEGF）和巨噬细胞炎症蛋白（MIP）-3α水平显著升高；而干细胞因子（SCF）水平显著降低。晚期疾病患者的 IL-6、IL-8、IP-10 和 MIP-3α 水平显著升高，且与 EBV DNA 相关。由 MIP-3α 和 SCF 组成的 2 个标志物组合增加了 EBV 特异性病毒壳抗原（VCA）IgA 或 EBV DNA 的筛查效果，尽管将 MIP-3α 和 SCF 添加到目前可用的标志物（EBV VCA IgA 和 EBV DNA）联合组中并不能提高诊断标志物组合的准确性。IL-8、VEGF、MIP-3α 和 EBV DNA 水平较高的 NPC 患者的总生存预后较差（p=0.008、0.035、0.005 和 0.007）。
同时进行多种细胞因子的大规模测量可能会提高 NPC 的检测和预后预测能力。
