United States Department of Agriculture-Agricultural Research Service and Department of Crop Sciences, University of Illinois, Urbana 61801.
Phytopathology. 2011 Jun;101(6):750-6. doi: 10.1094/PHYTO-09-10-0239.
Infection of soybean plants with Soybean mosaic virus (SMV), which is transmitted by aphids and through seed, can cause significant reductions in seed production and quality. Because seedborne infections are the primary sources of inoculum for SMV infections in North America, host-plant resistance to seed transmission can limit the pool of plants that can serve as sources of inoculum. To examine the inheritance of SMV seed transmission in soybean, crosses were made between plant introductions (PIs) with high (PI88799), moderate (PI60279), and low (PI548391) rates of transmission of SMV through seed. In four F(2) populations, SMV seed transmission segregated as if conditioned by two or more genes. Consequently, a recombinant inbred line population was derived from a cross between PIs 88799 and 548391 and evaluated for segregation of SMV seed transmission, seed coat mottling, and simple sequence repeat markers. Chromosomal regions on linkage groups C1 and C2 were significantly associated with both transmission of isolate SMV 413 through seed and SMV-induced seed coat mottling, and explained ≈42.8 and 46.4% of the variability in these two traits, respectively. Chromosomal regions associated with seed transmission and seed coat mottling contained homologues of Arabidopsis genes DCL3 and RDR6, which encode enzymes involved in RNA-mediated transcriptional and posttranscriptional gene silencing.
感染大豆花叶病毒（SMV）的大豆植株，其传播途径为蚜虫和种子，会导致种子产量和质量的显著下降。由于种传感染是北美 SMV 感染的主要接种源，因此宿主植物对种子传播的抗性可以限制作为接种源的植物群体。为了研究大豆花叶病毒种子传播的遗传，对种传率较高（PI88799）、中等（PI60279）和较低（PI548391）的植物引种（PI）进行了杂交。在四个 F2 群体中，SMV 种子传播的遗传符合两个或多个基因的条件。因此，从 PI88799 和 PI548391 之间的杂交中衍生出了一个重组自交系群体，并对其进行了 SMV 种子传播、种皮斑驳和简单重复序列标记的分离评估。连锁群 C1 和 C2 上的染色体区域与通过种子传播的分离株 SMV413 和 SMV 诱导的种皮斑驳显著相关，分别解释了这两个性状变异性的约 42.8%和 46.4%。与种子传播和种皮斑驳相关的染色体区域包含拟南芥基因 DCL3 和 RDR6 的同源物，这些基因编码参与 RNA 介导的转录和转录后基因沉默的酶。
