Graduate Institute of Engineering, Department of Materials Science and Engineering, National Taiwan University of Science and Technology, Taipei, 106, Taiwan, ROC.
Colloids Surf B Biointerfaces. 2011 Sep 1;86(2):409-13. doi: 10.1016/j.colsurfb.2011.04.002. Epub 2011 Apr 9.
In this study, a novel layered hydrogel composing of poly (γ-glutamic acid) (PGA), chitosan (CS), and alginate (AL) were prepared. Furthermore, PGA, CS, and AL were labeled with different fluorescent dyes. The bilayer structure of hydrogel was then revealed using these fluorescent labeled polymers. To mimic the stability of these hydrogels in physiological fluids, the dissolution of PGA and the release of Ca2+ from these hydrogels in normal saline were also monitored. The results showed that by adding CS to the hydrogel, the dissolution of PGA was decreased by 67%, and the release of Ca2+ was reduced by 40%. In addition, the hydrogel exhibited no cytotoxicity for L929 fibroblasts.
在这项研究中，制备了一种由聚（γ-谷氨酸）（PGA）、壳聚糖（CS）和海藻酸钠（AL）组成的新型层状水凝胶。此外，PGA、CS 和 AL 被标记上了不同的荧光染料。然后使用这些荧光标记的聚合物来揭示水凝胶的双层结构。为了模拟这些水凝胶在生理流体中的稳定性，还监测了 PGA 在生理盐水中的溶解和 Ca2+从这些水凝胶中的释放。结果表明，向水凝胶中添加 CS 后，PGA 的溶解减少了 67%，Ca2+的释放减少了 40%。此外，该水凝胶对 L929 成纤维细胞无细胞毒性。
