Dragunow M, Faull R L, Jansen K L
Department of Anatomy, School of Medicine, University of Auckland, New Zealand.
Neurosci Lett. 1990 Feb 5;109(1-2):128-33. doi: 10.1016/0304-3940(90)90550-s.
Unilateral lesions of the rat hippocampus produced by needle insertion lead to ipsilateral accumulation of c-fos protein in dentate granule cells and neurons in the piriform cortex, as well as in glial-like cells in the corpus callosum and in ependymal cells lining the lateral ventricle adjacent to the lesion site. C-fos protein was detected immunocytochemically using two different antibodies in formalin-fixed brain sections. The N-methyl-D-aspartate (NMDA) antagonist MK-801 produced a dose- and time-dependent inhibition of c-fos protein accumulation in dentate granule cells and in neurons in the piriform cortex, but did not affect glial or ependymal c-fos protein accumulation. MK-801 at 4 mg/kg injected two hours before lesion inhibited c-fos accumulation. Thus, c-fos protein accumulation in hippocampal neurons and in neurons in the piriform cortex induced after traumatic brain injury involves activation of NMDA receptors.
通过针刺造成大鼠海马体单侧损伤，会导致同侧齿状颗粒细胞、梨状皮质中的神经元以及胼胝体中的胶质样细胞和损伤部位附近侧脑室衬里的室管膜细胞中c-fos蛋白的积累。在福尔马林固定的脑切片中，使用两种不同的抗体通过免疫细胞化学方法检测到了c-fos蛋白。N-甲基-D-天冬氨酸（NMDA）拮抗剂MK-801对齿状颗粒细胞和梨状皮质中的神经元中c-fos蛋白的积累产生剂量和时间依赖性抑制，但不影响胶质细胞或室管膜细胞中c-fos蛋白的积累。损伤前两小时注射4mg/kg的MK-801可抑制c-fos的积累。因此，创伤性脑损伤后诱导的海马神经元和梨状皮质神经元中c-fos蛋白的积累涉及NMDA受体的激活。
