Antonov S M, Kalinina N I, Kurchavyj G G, Magazanik L G, Shupliakov O V, Vesselkin N P
Sechenov Institute of Evolutionary Physiology and Biochemistry, Academy of Sciences of the USSR, Leningrad.
Neurosci Lett. 1990 Feb 5;109(1-2):82-7. doi: 10.1016/0304-3940(90)90541-g.
Synaptic responses induced in motoneurones by stimulation of dorsal root (DR) and reticular formation (RF) were recorded intracellularly in the isolated frog spinal cord. Argiopine (spider neurotoxin, a selective blocker of glutamate receptors of the non-N-methyl-D-aspartate (non-NMDA) type) in concentrations from 3 x 10(-7) to 1 x 10(-5) M effectively inhibited di- and polysynaptic components of excitatory postsynaptic potentials (EPSPs) induced by DR and RF stimulation. The monosynaptic component of the RF response was inhibited by argiopine, whereas the monosynaptic component of DR response was actually enhanced. All types of EPSPs studied were blocked by kynurenate (1-2 x 10(-3) M). D,L-2-Amino-5-phosphonovaleric acid (1 x 10(-4) M) slightly diminished the amplitude only of polysynaptic DR responses. A special type of argiopine-resistant, non-NMDA glutamate receptor is assumed to be involved in the generation of an EPSP by the monosynaptic DR input.
在离体蛙脊髓中，通过细胞内记录法记录了刺激背根（DR）和网状结构（RF）在运动神经元中诱发的突触反应。浓度为3×10⁻⁷至1×10⁻⁵M的阿吉奥平（蜘蛛神经毒素，一种非N-甲基-D-天冬氨酸（非NMDA）型谷氨酸受体的选择性阻断剂）有效抑制了DR和RF刺激诱发的兴奋性突触后电位（EPSP）的双突触和多突触成分。RF反应的单突触成分被阿吉奥平抑制，而DR反应的单突触成分实际上增强。所研究的所有类型的EPSP均被犬尿烯酸（1-2×10⁻³M）阻断。D，L-2-氨基-5-磷酸戊酸（1×10⁻⁴M）仅略微降低了多突触DR反应的幅度。推测一种特殊类型的抗阿吉奥平非NMDA谷氨酸受体参与了单突触DR输入诱发EPSP的产生。
