精蜘蛛肽可阻断离体蛙脊髓运动神经元中的谷氨酸反应和感觉运动传递。

Argiopin blocks the glutamate responses and sensorimotor transmission in motoneurones of isolated frog spinal cord.

作者信息

Antonov S M, Grishin E V, Magazanik L G, Shupliakov O V, Vesselkin N P, Volkova T M

机构信息

Sechenov Institute of Evolutionary Physiology and Biochemistry, Academy of Sciences, Leningrad, U.S.S.R.

出版信息

Neurosci Lett. 1987 Dec 16;83(1-2):179-84. doi: 10.1016/0304-3940(87)90237-0.

DOI:10.1016/0304-3940(87)90237-0

PMID:2894622

Abstract

Argiopin, a low-molecular weight component of the spider Argiope lobata venom, inhibited depolarizations of motoneurones induced by glutamate in experiments on the frog isolated spinal cord, but had no effect on aspartate-induced responses. Half of the blocking effect (ED50) was seen at 7.5 +/- 3.7 x 10(-8) M argiopin. The same concentrations of argiopin (7.5 x 10(-8) M to 2.3 x 10(-7) M) suppressed the responses of the ventral root to electrical stimulation of the dorsal root. The results suggest that argiopin selectively blocks only one population of the excitatory amino acid receptors on motoneurones, and these argiopin-sensitive receptors are found to be involved in sensorimotor synaptic transmission in the spinal cord.

摘要

银斑蛛毒素是银斑蛛毒液中的一种低分子量成分，在青蛙离体脊髓实验中，它能抑制由谷氨酸诱导的运动神经元去极化，但对天冬氨酸诱导的反应没有影响。在7.5±3.7×10⁻⁸ M的银斑蛛毒素浓度下可观察到半数阻断效应（ED50）。相同浓度的银斑蛛毒素（7.5×10⁻⁸ M至2.3×10⁻⁷ M）可抑制腹根对背根电刺激的反应。结果表明，银斑蛛毒素仅选择性阻断运动神经元上的一群兴奋性氨基酸受体，并且发现这些对银斑蛛毒素敏感的受体参与脊髓的感觉运动突触传递。