超螺旋DNA中的B-A转变

The B-A transition in superhelical DNA.

作者信息

Makarov V L, Ivanov V I

机构信息

Engelhardt Institute of Molecular Biology, USSR Academy of Sciences, Moscow.

出版信息

Nucleic Acids Res. 1990 Feb 25;18(4):759-61. doi: 10.1093/nar/18.4.759.

DOI:10.1093/nar/18.4.759

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC330324/

Abstract

Relaxation of a DNA superhelical stress due to the B to A transition induced by trifluoroethanol has been studied by assessing the change of DNA orientation in a flow gradient. Using DNAs of different superhelical densities, a decrease in the winding angle during the B----A shift of DNA was found to be 1.5 degrees per base pair in solution. Accepting the winding angle for B-DNA in solution to be 34.1 degrees, that for A-DNA must have a value of 32.6 degrees which agrees with the X-ray data for A-DNA in the condensed state. The date obtained within the B-A transition interval make it possible to conclude that there is an increase in winding at each B/A junction, which is about 5 degrees per one junction.

摘要

通过评估在流动梯度中DNA取向的变化，研究了三氟乙醇诱导的B型到A型转变导致的DNA超螺旋应力的松弛。使用不同超螺旋密度的DNA，发现在溶液中DNA从B型转变为A型时，每碱基对的缠绕角减小1.5度。假设溶液中B-DNA的缠绕角为34.1度，那么A-DNA的缠绕角必定为32.6度，这与凝聚态A-DNA的X射线数据相符。在B-A转变区间内获得的数据使得可以得出结论，即在每个B/A连接处缠绕增加，每个连接处约为5度。

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