Goulet I, Zivanovic Y, Prunell A
Nucleic Acids Res. 1987 Apr 10;15(7):2803-21. doi: 10.1093/nar/15.7.2803.
The V-like dependence of the electrophoretic mobility of small DNA rings on their topological constraint, which has been documented in a recent paper [Zivanovic et al. (1986), J. Mol. Biol., 192, 645-660], has been explored as a tool to measure the helical twist of the torsionally unstressed duplex. The method was applied to single mixed sequence fragments approximately 350 to 1400 base pairs in length, providing estimates of their average helical periodicity. It was also used to compare two DNA fragments, and to evaluate the helical repeat of poly(dA.dT).poly(dA.dT) and poly(dA).poly(dT) inserts, and the helix unwindings associated with dA and dC methylations by dam and Hhal methylases, respectively. Data were found to be highly reproducible and helical repeat estimates were in good agreement with those obtained from previous techniques.
小DNA环的电泳迁移率对其拓扑约束的V型依赖性，已在最近一篇论文中得到记录[齐瓦诺维奇等人（1986年），《分子生物学杂志》，192卷，645 - 660页]，该依赖性已被探索作为一种测量无扭转应力双链体螺旋扭曲的工具。该方法应用于长度约为350至1400个碱基对的单个混合序列片段，提供了它们平均螺旋周期的估计值。它还被用于比较两个DNA片段，评估聚（dA.dT）·聚（dA.dT）和聚（dA）·聚（dT）插入片段的螺旋重复，以及分别与dam和Hhal甲基化酶介导的dA和dC甲基化相关的螺旋解旋。发现数据具有高度可重复性，并且螺旋重复估计值与先前技术获得的结果高度一致。
