通过改良的环介导等温扩增法检测丙型肝炎病毒。
Detection of hepatitis C virus by an improved loop-mediated isothermal amplification assay.
机构信息
State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Institute of Infectious Diseases, The First Affiliated Hospital of Zhejiang University, School of Medicine, Zhejiang University, Hangzhou, 310022, China.
出版信息
Arch Virol. 2011 Aug;156(8):1387-96. doi: 10.1007/s00705-011-1001-4. Epub 2011 May 12.
An improved, sensitive, specific, and rapid one-step reverse transcription loop-mediated isothermal amplification (LAMP) assay targeting the 5' untranslated region (UTR) was developed to detect hepatitis C virus (HCV) infection. Based on an accelerating primer (AP), the present assay, named AP-LAMP, has the advantages of rapidity and sensitivity over the routine LAMP method. The possible AP-based amplification pathway during the reaction was revealed by restriction enzyme digestion and eletrophoresis. The detection limit of the AP-LAMP assay was approximately 84 IU/ml, and no cross-detection was observed. The assay was evaluated further with 126 clinical specimens, and the results indicated the suitability and simplicity of the test as a rapid diagnostic tool for detection of HCV RNA.
一种改良的、敏感的、特异的、快速的一步逆转录环介导等温扩增（LAMP）检测方法，针对 5'非翻译区（UTR），用于检测丙型肝炎病毒（HCV）感染。基于加速引物（AP），本检测方法，命名为 AP-LAMP，在快速性和灵敏度方面优于常规 LAMP 方法。通过酶切和电泳揭示了反应过程中可能的基于 AP 的扩增途径。AP-LAMP 检测的检测限约为 84IU/ml，没有交叉检测。该检测方法进一步用 126 个临床标本进行了评估，结果表明该检测方法作为 HCV RNA 的快速诊断工具具有适用性和简单性。
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