Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan 610015, China.
J Tradit Chin Med. 2011 Mar;31(1):21-6. doi: 10.1016/s0254-6272(11)60005-8.
To study the drug property differences of Shexiang (Moschus) and Bingpian (Borneolum Synthcticum) with biothermodynamics, and to verify the objectivity and authenticity of the drug property.
The growth-thermogram curves of Escherichia coli (E. coli) affected by Shexiang (Moschus) and Bingpian (Borneolum Synthcticum) at different concentrations were determined with microcalorimetry, and the power-time curves (thermogram curves) of E. coli metabolism and characteristic parameters, such as growth rate constant (k), maximum output power (P(m)), peak time (t(p)), total heat-output (Q(t)), etc. were analyzed with the principal component analysis (PCA) to find the close correlative parameters, so as to objectively reflect the drug property differences of Shexiang (Moschus) and Bingpian (Borneolum Synthcticum).
The values of P2 in the second exponential growth phase increased with the increase of the concentrations of Shexiang (Moschus) and Bingpian (Borneolum Synthcticum), and the P2 of Shexiang (Moschus) was larger than that of Bingpian (Borneolum Synthcticum); Q2 increased with the increase of the concentrations of Shexiang (Moschus), but for Bingpian (Borneolum Synthcticum) it was opposite. It is indicated that they have different effects on P2 and Q2 of E. coli in the second exponential growth phase, and have differences in warm and cold natures.
The microcalorimetry can accurately and objectively appraise differences of the drug property of Shexiang (Moschus) and Bingpian (Borneolum Synthcticum) and verify the objectivity and authenticity of the drug property, so as to provide a new and useful method for studies of the drug property of Chinese drugs.
应用生物热动力学方法研究麝香与人工合成冰片药性的差异，验证药性的客观性和真实性。
应用微量热法测定不同浓度麝香与人工合成冰片对大肠杆菌作用的生长热谱曲线，用主成分分析（PCA）对大肠杆菌代谢的功率-时间曲线（热谱曲线）及其特征参数，如比生长速率常数（k）、最大输出功率（Pm）、峰值时间（tp）、总热量输出（Qt）等进行分析，寻找密切相关参数，客观反映麝香与人工合成冰片药性的差异。
在第二对数生长期，P2 值随麝香与人工合成冰片浓度的增加而增大，麝香的 P2 值大于人工合成冰片；Q2 值随麝香浓度的增加而增大，而人工合成冰片则相反。表明它们对大肠杆菌第二对数生长期的 P2 和 Q2 有不同的影响，具有寒热药性的差异。
微量热法能准确、客观地评价麝香与人工合成冰片药性的差异，验证了药性的客观性和真实性，为中药药性研究提供了新的、有用的方法。
