Soussi B, Idström J P, Scherstén T, Bylund-Fellenius A C
Department of Surgery, University of Gothenburg, Sweden.
Acta Physiol Scand. 1990 Feb;138(2):107-14. doi: 10.1111/j.1748-1716.1990.tb08822.x.
The effect of 2 and 4 h of tourniquet ischaemia followed by 1 h of reperfusion on the major mitochondrial phospholipids and on the cytochrome c oxidase kinetic parameters has been investigated in rat skeletal muscle. There was no change either in the mitochondrial phospholipid content or in the Vmax and the Km of the enzyme after 2 h of ischaemia with and without subsequent reperfusion. Four hours of ischaemia had no effect on the lecithin and the cephalin content, while the cardiolipin content decreased as well as the Vmax of the enzyme (P less than 0.05). Tissue reperfusion caused a dramatic decrease in both cardiolipin (55% of the control, P less than 0.001) and Vmax (38% of the control, P less than 0.001). The corresponding reduction in lecithin and cephalin contents was 12% and 14% respectively (P less than 0.05). The Km remained unchanged at all conditions. These findings suggest that mitochondrial dysfunction in response to ischaemia and reperfusion could be a consequence of the reperfusion itself following severe ischaemia. The results are discussed in terms of cardiolipin peroxidation and cytochrome oxidase as a functional parameter.
在大鼠骨骼肌中，研究了2小时和4小时的止血带缺血再灌注1小时对主要线粒体磷脂和细胞色素c氧化酶动力学参数的影响。缺血2小时无论随后有无再灌注，线粒体磷脂含量、酶的最大反应速度（Vmax）和米氏常数（Km）均无变化。缺血4小时对卵磷脂和脑磷脂含量无影响，但心磷脂含量降低，酶的Vmax也降低（P<0.05）。组织再灌注导致心磷脂（降至对照组的55%，P<0.001）和Vmax（降至对照组的38%，P<0.001）急剧下降。卵磷脂和脑磷脂含量相应分别降低12%和14%（P<0.05）。在所有条件下，Km均保持不变。这些发现表明，缺血再灌注引起的线粒体功能障碍可能是严重缺血后再灌注本身的结果。从心磷脂过氧化和细胞色素氧化酶作为功能参数的角度对结果进行了讨论。
