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黑刺尾鬣蜥(Ctenosaura pectinata)多态微卫星标记的分离与特征分析及其在其他 Ctenosaura 中的交叉实用性。

Isolation and characterization of polymorphic microsatellite markers in the black spiny tailed iguana (Ctenosaura pectinata) and their cross-utility in other Ctenosaura.

机构信息

School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, UK, Colección Nacional de Anfibios y Reptiles, Instituto de Biología, Universidad Nacional Autónoma de México, Ciudad Universitaria, D.F., CP 04510, México.

出版信息

Mol Ecol Resour. 2009 Jan;9(1):117-9. doi: 10.1111/j.1755-0998.2008.02379.x.

DOI:10.1111/j.1755-0998.2008.02379.x
PMID:21564576
Abstract

We isolated and characterized 10 polymorphic microsatellite loci from the Mexican black iguana (Ctenosaura pectinata) and assessed levels of polymorphism in sampling sites located in the northern areas of the species' distribution range. Two to 19 alleles per locus and observed heterozygosity ranging from 0.15 to 0.96 were detected. These markers will be useful to describe population genetic structure, the extent of gene flow in contact zones, to study the mating system of the species and to address conservation genetics issues. Additionally, we evaluated the potential utility of these markers for studies of other species within the genus Ctenosaura (i.e. C. hemilopha, C. similis and C. oaxacana).

摘要

我们从墨西哥黑鬣蜥(Ctenosaura pectinata)中分离并鉴定了 10 个多态性微卫星位点,并评估了分布范围北部采样点的多态性水平。每个位点检测到 2 到 19 个等位基因,观察到的杂合度从 0.15 到 0.96 不等。这些标记物将有助于描述种群遗传结构、接触区基因流的程度、研究物种的交配系统以及解决保护遗传学问题。此外,我们还评估了这些标记物在研究 Ctenosaura 属内其他物种(即 C. hemilopha、C. similis 和 C. oaxacana)中的潜在应用。

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引用本文的文献

1
Introgressive hybridization in a Spiny-Tailed Iguana, , and its implications for taxonomy and conservation.一只刺尾鬣蜥的渐渗杂交及其对分类学和保护的影响。
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