南方澳无须鳕微卫星位点的分离与特征分析及其跨物种扩增。

Isolation and characterization of microsatellite loci in Merluccius australis and cross-species amplification.

机构信息

Departamento Biologia Funcional, Facultad de Medicina, Universidad de Oviedo C/J, Claveria s/n, 33006-Oviedo, Spain.

出版信息

Mol Ecol Resour. 2009 Mar;9(2):585-7. doi: 10.1111/j.1755-0998.2008.02442.x. Epub 2009 Jan 31.

DOI:10.1111/j.1755-0998.2008.02442.x

Abstract

Eight novel and two heterologous microsatellite pairs of primers are presented for the Austral hake (Merluccius australis), representing the first microsatellite markers available for this species. Loci were characterized for 50 individuals from two populations in South America (Argentinean and Chilean coasts). All loci were polymorphic within M. australis (5 to 30 alleles per locus; observed heterozygosity between 0.320 and 0.840), and therefore useful for population genetic studies within the species. Cross-species transferability was tested for 100 individuals from four additional species within the Merluccius genus (M. albidus, M. bilinearis, M. gayi and M. hubbsi), and results indicate that most of these primers pairs will likely be useful for population genetic studies on Merluccius species.

摘要

现提供 8 对新的和 2 对异源微卫星引物，用于南方无须鳕（Merluccius australis），这是该物种可用的第一批微卫星标记。在来自南美洲（阿根廷和智利海岸）的两个种群的 50 个个体中对这些位点进行了特征描述。所有位点在南方无须鳕内均具有多态性（每个位点 5 至 30 个等位基因；观察到的杂合度在 0.320 至 0.840 之间），因此可用于该物种的种群遗传研究。对来自 Merluccius 属的另外 4 个物种的 100 个个体进行了跨物种可转移性测试（M. albidus、M. bilinearis、M. gayi 和 M. hubbsi），结果表明，这些引物对中的大多数可能将有助于 Merluccius 物种的种群遗传研究。