从澳大利亚短鳍鳗（Anguilla australis Richardson）中分离和鉴定多态性微卫星标记。

Isolation and characterization of polymorphic microsatellite markers from Australia short-finned eel (Anguilla australis Richardson).

机构信息

Key Laboratory of Aquatic Genetic Resources and Aquacultural Ecosystem Certificated by the Ministry of Agriculture, Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Shanghai Fisheries University, Ministry of Education, No. 334 Jungong Road, Shanghai 200090, China.

出版信息

Mol Ecol Resour. 2008 Jul;8(4):777-9. doi: 10.1111/j.1755-0998.2007.02060.x.

DOI:10.1111/j.1755-0998.2007.02060.x

PMID:21585889

Abstract

Seventeen microsatellite DNA loci from the Australian short-finned eel (Anguilla australis Richardson) were isolated and their amplification characteristics were described. The polymerase chain reaction primers were tested on 40 eel individuals. The primers amplified loci with relatively high numbers of alleles, ranging from five to 14 with an average of nine per locus. Mean observed heterozygosity (H(O) ) and expected heterozygosity (H(E) ) were 0.6779 and 0.7374, respectively, indicating that these markers would be useful for population studies. No loci deviated significantly from Hardy-Weinberg equilibrium (P = 0.05) and no evidence was found for genotypic disequilibrium among loci at a 5% significance level.

摘要

从澳大利亚短鳍鳗（Anguilla australis Richardson）中分离出 17 个微卫星 DNA 位点，并描述了它们的扩增特征。对 40 条鳗鱼个体进行了聚合酶链反应引物测试。引物扩增出具有相对较多等位基因的位点，每个位点的等位基因数从 5 到 14 不等，平均为 9 个。观测杂合度（H(O)）和期望杂合度（H(E)）分别为 0.6779 和 0.7374，表明这些标记物将对种群研究有用。没有位点显著偏离哈迪-温伯格平衡（P=0.05），也没有发现 5%显著水平下的位点间基因型不平衡。