用于蓝圆鲹（Scomber australasicus）种群遗传研究的微卫星 DNA 标记及在日本竹荚鱼（S. japonicus）中的种间特异性扩增。

Microsatellite DNA markers for population-genetic studies of blue mackerel (Scomber australasicus) and cross-specific amplification in S. japonicus.

机构信息

Research Centre for Biodiversity, Academia Sinica, Nankang, Taipei 115, Taiwan Institute of Zoology, Department of Life Science, National Taiwan University, Taipei 106, Taiwan Institute of Marine Affairs and Resource Management, National Taiwan Ocean University, Keelung 20224, Taiwan.

出版信息

Mol Ecol Resour. 2009 May;9(3):824-7. doi: 10.1111/j.1755-0998.2008.02278.x. Epub 2009 Jan 22.

DOI:10.1111/j.1755-0998.2008.02278.x

PMID:21564757

Abstract

Blue mackerel (Scomber australasicus) is targeted by large-scale purse-seiners in the western North Pacific, and its stock structure is still contentious. Herein, we described 10 polymorphic microsatellite loci for blue mackerel. The number of alleles among 32 individuals surveyed ranged from five to 27 (average of 16.2 alleles per locus). Departures from Hardy-Weinberg expectation were observed at two loci. Cross-specific amplification in the congener, S. japonicus, was successful, except for one locus, revealed to be diagnostic for these congeners. These microsatellite loci will be useful tools to address queries in population genetic structure, fishery management unit and taxonomic species status in the genus Scomber.

摘要

蓝圆鲹（Scomber australasicus）是西北太平洋大型围网渔船的捕捞对象，其种群结构仍存在争议。本文描述了蓝圆鲹的 10 个多态微卫星位点。在所调查的 32 个个体中，等位基因数从 5 到 27 个不等（每个位点平均 16.2 个等位基因）。有两个位点偏离了哈迪-温伯格期望。在同属的竹荚鱼（S. japonicus）中，除了一个位点外，其他位点的交叉特异性扩增均成功，该位点可用于这些同属鱼类的鉴定。这些微卫星位点将是解决蓝圆鲹属种群遗传结构、渔业管理单位和分类物种地位等问题的有用工具。