日本鳀（鳀科）的多态微卫星位点

Polymorphic microsatellite loci for the Japanese anchovy Engraulis japonicus (Engraulidae).

作者信息

Lin L, Zhu L, Liu S-F, Su Y-Q, Zhuang Z-M

机构信息

College of Oceanography and Environmental Science, Xiamen University, Xiamen, China.

出版信息

Genet Mol Res. 2011 May 3;10(2):764-8. doi: 10.4238/vol10-2gmr1085.

DOI:10.4238/vol10-2gmr1085

PMID:21563070

Abstract

We isolated and characterized 14 polymorphic microsatellite loci in the Japanese anchovy (Engraulis japonicus) using a (GT)(13)-enriched genomic library. The numbers of alleles per locus ranged from 6 to 31, with a mean of 17.8. The observed and expected heterozygosities ranged from 0.180 to 0.949 and from 0.172 to 0.966, with means of 0.731 and 0.825, respectively. All 14 loci were in Hardy-Weinberg equilibrium and no significant linkage disequilibrium between loci pairs was detected. These microsatellite markers will be useful for analyzing the population genetic structure and gene flow of E. japonicus.

摘要

我们利用一个富含(GT)(13)的基因组文库，分离并鉴定了日本鳀（Engraulis japonicus）中的14个多态微卫星位点。每个位点的等位基因数从6到31不等，平均为17.8。观察到的杂合度和预期杂合度分别在0.180至0.949和0.172至0.966之间，平均值分别为0.731和0.825。所有14个位点均处于哈迪-温伯格平衡，且未检测到位点对之间存在显著的连锁不平衡。这些微卫星标记将有助于分析日本鳀的种群遗传结构和基因流动。