Departamento de Biologia Geral, Universidade Federal de Viçosa, Viçosa, MG, Brazil.
Mol Ecol Resour. 2009 May;9(3):923-5. doi: 10.1111/j.1755-0998.2008.02486.x. Epub 2009 Jan 22.
Eight microsatellite primers were developed from ISSR (intersimple sequence repeats) markers for the stingless bee Melipona rufiventris. These primers were tested in 20 M. rufiventris workers, representing a single population from Minas Gerais state. The number of alleles per locus ranged from 2 to 5 (mean = 2.63) and the observed and expected heterozygosity values ranged from 0.00 to 0.44 (mean = 0.20) and from 0.05 to 0.68 (mean = 0.31), respectively. Several loci were also polymorphic in M. quadrifasciata, M. bicolor, M. mandacaia and Partamona helleri and should prove useful in population studies of other stingless bees.
从 ISSR（简单重复序列间）标记中为无刺蜂 Melipona rufiventris 开发了 8 个微卫星引物。这些引物在 20 只 M. rufiventris 工蜂中进行了测试，代表了来自米纳斯吉拉斯州的单一种群。每个位点的等位基因数范围为 2 到 5（平均值为 2.63），观察到的和预期的杂合度值范围分别为 0.00 到 0.44（平均值为 0.20）和 0.05 到 0.68（平均值为 0.31）。M. quadrifasciata、M. bicolor、M. mandacaia 和 Partamona helleri 中的几个位点也呈现多态性，这些引物应该对其他无刺蜂种群研究有用。
