Francisco Flávio de O, Brito Rute M, Arias Maria C
Depto. Genética e Biologia Evolutiva, Instituto de Biociências, Univ. São Paulo, 05508-090, São Paulo, SP.
Neotrop Entomol. 2006 Sep-Oct;35(5):638-43. doi: 10.1590/s1519-566x2006000500011.
In the present study we compare genetic characteristics (allele diversity and observed heterozygosity) of microsatellite loci, from three stingless bee species (Plebeia remota Holmberg, Partamona mulata Moure In Camargo and Partamona helleri Friese), amplified by using heterospecific primers originally designed for Melipona bicolor Lepeletier and Scaptotrigona postica Latreille. We analyzed 360 individuals of P. remota from 72 nests, 58 individuals of R. mulata from 58 nests, and 47 individuals of P. helleri from 47 nests. The three species studied showed low level of polymorphism for the loci amplified with primers derived from M. bicolor. However, for the loci amplified with primers derived from S. postica, only P. remota presented low level of polymorphism.
在本研究中，我们比较了三种无刺蜂（Plebeia remota Holmberg、Partamona mulata Moure In Camargo和Partamona helleri Friese）微卫星位点的遗传特征（等位基因多样性和观察到的杂合度），这些微卫星位点是使用最初为双色无刺蜂（Melipona bicolor Lepeletier）和后足无刺蜂（Scaptotrigona postica Latreille）设计的异源特异性引物进行扩增的。我们分析了来自72个巢穴的360只P. remota个体、来自58个巢穴的58只R. mulata个体以及来自47个巢穴的47只P. helleri个体。研究的这三个物种在用源自双色无刺蜂的引物扩增的位点上表现出低水平的多态性。然而，在用源自后足无刺蜂的引物扩增的位点上，只有P. remota表现出低水平的多态性。
