来自炭腐病菌（Macrophomina phaseolina）的微卫星。

Microsatellites from the charcoal rot fungus (Macrophomina phaseolina).

机构信息

Entomology and Plant Pathology, Mississippi State University, Starkville, MS 39762, USA, Entomology and Plant Pathology, University of Tennessee, Knoxville, TN 37996, USA, USDA-ARS Southern Horticultural Laboratory, Poplarville, MS 39470, USA, USDA-ARS-CGRU MSA, Stoneville, MS 38776, USA, University of Minnesota, Minneapolis, MN 54455 USA.

出版信息

Mol Ecol Resour. 2009 May;9(3):946-8. doi: 10.1111/j.1755-0998.2008.02511.x. Epub 2009 Mar 2.

DOI:10.1111/j.1755-0998.2008.02511.x

PMID:21564800

Abstract

Microsatellite loci were identified from the charcoal rot fungus (Macrophomina phaseolina). Primer pairs for 46 loci were developed, and of these, 13 were optimized and screened using genomic DNA from 55 fungal isolates collected predominantly from two soybean fields in Mississippi. Twelve of the optimized loci were polymorphic and the number of alleles per locus ranged from 6 to 22. These microsatellites will be useful in population and pathogenicity studies to correspond with development of potential disease-resistant soybean and other susceptible crops.

摘要

从炭腐病菌（Macrophomina phaseolina）中鉴定出微卫星位点。开发了 46 对引物，其中 13 对使用主要从密西西比州的两个大豆田中收集的 55 个真菌分离株的基因组 DNA 进行了优化和筛选。优化后的 12 个位点具有多态性，每个位点的等位基因数范围为 6 至 22。这些微卫星标记将在种群和致病性研究中有用，以对应潜在的抗病大豆和其他易感作物的开发。