Entomology and Plant Pathology, University of Tennessee, Knoxville, TN 37996, USA, USDA-ARS-CGRU MSA Genomics Laboratory, 141 Experiment Station Road, Stoneville, MS 38776, USA, USDA-ARS Southern Horticultural Laboratory, 810 Highway 26 West, Poplarville, MS 39470, USA.
Mol Ecol Resour. 2008 Jul;8(4):780-2. doi: 10.1111/j.1755-0998.2007.02062.x.
Microsatellite loci were identified from Cornus kousa'National'. Primer pairs for 86 loci were developed and of these, eight were optimized and screened using genomic DNA from 22 kousa cultivars. All optimized loci were polymorphic and the number of alleles per locus ranged from three to 17. Observed heterozygosity ranged from 0 to 0.3 and expected heterozygosity ranged from 0.38 to 0.91. These microsatellites will be useful in population studies, and a breeding programme for cultivar development of Cornus species.
从‘National’山茱萸中鉴定出微卫星位点。为 86 对引物开发了引物对，其中 8 对使用 22 个山茱萸品种的基因组 DNA 进行了优化和筛选。所有优化的位点都是多态的，每个位点的等位基因数从 3 到 17 不等。观察到的杂合度范围从 0 到 0.3，预期杂合度范围从 0.38 到 0.91。这些微卫星标记将有助于种群研究，以及山茱萸品种选育的育种计划。
