Key Laboratory of Aquatic Genetic Resources and Aquacultural Ecosystem, Ministry of Agriculture, Shanghai Ocean University, 999 Hu Cheng Ring Road, Shanghai 201306, China.
Mol Ecol Resour. 2009 May;9(3):971-4. doi: 10.1111/j.1755-0998.2009.02520.x. Epub 2009 Feb 4.
A rapid method for isolating microsatellite loci in blunt snout bream, based on the 5'-anchored polymerase chain reaction technique, revealed 522 microsatellite loci (consisting of 442 dinucleotide, 4 trinucleotide and 76 tetranucleotide repeats). Of the 25 loci characterized, 10 turned out to be highly polymorphic. The number of alleles per locus ranged from 3 to 17 while the expected heterozygosity ranged from 0.4899 to 0.9355 in population of selected strain F(7 ) and from 0.5786 to 0.9556 in wild population from Lake Liangzi. These markers are useful as tools for the detection of genetic variation levels in selected strains and wild populations of blunt snout bream for germplasm conservation.
一种基于 5'-锚定聚合酶链反应技术的快速分离 blunt snout bream 微卫星基因座的方法，揭示了 522 个微卫星基因座（由 442 个二核苷酸、4 个三核苷酸和 76 个四核苷酸重复组成）。在所鉴定的 25 个基因座中，有 10 个表现出高度多态性。每个基因座的等位基因数范围为 3 到 17，而在所选 F(7)菌株的群体中，预期杂合度范围为 0.4899 到 0.9355，在梁子湖的野生群体中，预期杂合度范围为 0.5786 到 0.9556。这些标记物可作为检测所选菌株和野生群体遗传变异水平的工具，用于 blunt snout bream 的种质保存。
