Yao H F, Sun D Q, Wang R X, Shi G
Fishery School, Zhejiang Ocean University, Zhoushan, PR China.
Genet Mol Res. 2012 May 21;11(2):1503-6. doi: 10.4238/2012.May.21.7.
Scylla paramamosain is a widespread and commercially important species of coastal marine crab. We identified 13 polymorphic microsatellite loci from a genome library constructed with 5'-anchored PCR method. Thirty-two S. paramamosain from the East China Sea were used to analyze the characteristics of these loci. The number of alleles per locus ranged from 3 to 8, with a mean of 5.923. Observed and expected heterozygosities ranged from 0.500 to 0.875 and from 0.500 to 0.859, respectively. Eleven of the 13 loci were highly polymorphic (polymorphic information content >0.5). All of the 13 novel loci were in Hardy-Weinberg equilibrium after Bonferroni's correction (P < 0.0038). There was no null allele, stuttering errors or evidence of allelic dropout in any of the loci analyzed by MICRO-CHECKER. According to pairwise tests, no significant linkage disequilibrium was found among the 13 loci (P < 0.0038, adjusted value). These novel developed microsatellites will be useful for studies of genetic variation, population structure, conservation genetics, and molecular-assisted selective breeding of S. paramamosain.
锯缘青蟹是一种广泛分布且具有重要商业价值的沿海海洋蟹类。我们通过5'-锚定PCR方法构建的基因组文库中鉴定出13个多态性微卫星位点。利用来自东海的32只锯缘青蟹分析这些位点的特征。每个位点的等位基因数范围为3至8，平均为5.923。观察到的杂合度和期望杂合度分别在0.500至0.875和0.500至0.859之间。13个位点中的11个具有高度多态性（多态信息含量>0.5）。经Bonferroni校正后，所有13个新位点均处于Hardy-Weinberg平衡（P < 0.0038）。通过MICRO-CHECKER分析的任何位点均未发现无效等位基因、结巴错误或等位基因缺失的证据。根据成对检验，在13个位点之间未发现显著的连锁不平衡（P < 0.0038，校正值）。这些新开发的微卫星将有助于锯缘青蟹的遗传变异、种群结构、保护遗传学和分子辅助选择育种研究。
