NERC Molecular Genetics Facility, Department of Animal and Plant Sciences, University of Sheffield, Western Bank, Sheffield, South Yorkshire S10 2TN, UK.
Mol Ecol Resour. 2009 Jul;9(4):1255-8. doi: 10.1111/j.1755-0998.2009.02626.x. Epub 2009 Mar 12.
We have characterized 20 polymorphic microsatellite loci in the hihi Notiomystis cincta. Loci were identified by testing loci originally isolated in other avian species and by isolating new microsatellites from a hihi genomic library. These loci were characterized in unrelated hihi from a single population on Tiritiri Matangi Island (n = 98). Each locus displayed between two and 10 alleles, and observed heterozygosities ranged between 0.29 and 0.91. Nineteen of the 20 polymorphic loci could be assigned a chromosome location in the zebra finch, Taeniopygia guttata genome based on sequence homology.
我们对 20 个多态性微卫星位点进行了特征描述，这些位点存在于 hihi（Notiomystis cincta）中。通过对最初在其他鸟类物种中分离出的位点进行测试，以及从 hihi 基因组文库中分离出新的微卫星，从而鉴定出了这些位点。这些位点是在 Tiritiri Matangi 岛上的一个单一种群中，来自于无关的 hihi（n=98）中进行特征描述的。每个位点显示出 2 到 10 个等位基因，观察到的杂合度范围在 0.29 到 0.91 之间。基于序列同源性，在斑马雀（Taeniopygia guttata）基因组中，可将 20 个多态性位点中的 19 个分配到特定的染色体位置。
