Angus R, Lai T, Collins C, Stonebridge B, Symes M
UNIV BRISTOL,BRISTOL ROYAL INFIRM,DEPT SURG,BRISTOL BS2 8HW,AVON,ENGLAND. UNIV BRISTOL,BRISTOL ROYAL INFIRM,DEPT PATHOL,BRISTOL BS2 8HW,AVON,ENGLAND. UNIV BRISTOL,BRISTOL ROYAL INFIRM,DEPT COMP SCI,BRISTOL BS2 8HW,AVON,ENGLAND.
Int J Oncol. 1994 Jun;4(6):1383-6. doi: 10.3892/ijo.4.6.1383.
Specimens were obtained from the tumour and adjacent normal kidney in 29 patients with renal carcinoma. Expression of the alpha, pi and mu isoforms of gluthatione-S-transferase (GST) was estimated in formalin fixed paraffin embedded sections of the normal and neoplastic kidney following exposure to the appropriate polyclonal antibodies. binding of which was recognised by the indirect peroxidase technique. Expression of alpha, pi and mu GST in each tumour specimen was compared with the in vitro sensitivity of tumour cells to doxorubicin determined by drug inhibition of [Se-75] selenomethionine uptake by the appropriate tumour cells in culture. Expression of pi GST was associated with tumour cell resistance to doxorubicin. The level of alpha, pi and mu GST was not related to patient survival.
从29例肾癌患者的肿瘤组织及相邻正常肾组织获取标本。在甲醛固定石蜡包埋的正常肾和肿瘤肾切片中，使用相应的多克隆抗体估计谷胱甘肽 - S - 转移酶（GST）的α、π和μ同工型的表达，其结合通过间接过氧化物酶技术识别。将每个肿瘤标本中的α、π和μ GST表达与通过培养中适当肿瘤细胞对[Se - 75]硒蛋氨酸摄取的药物抑制作用所确定的肿瘤细胞对阿霉素的体外敏感性进行比较。π GST的表达与肿瘤细胞对阿霉素的耐药性相关。α、π和μ GST的水平与患者生存率无关。
