Isolation of a Rhodobacter capsulatus bioB mutant and cloning of the bioB gene.
作者信息
Cardin R D, Biel A J
机构信息
Department of Microbiology, Louisiana State University, Baton Rouge 70803.
出版信息
J Bacteriol. 1990 Apr;172(4):2181-3. doi: 10.1128/jb.172.4.2181-2183.1990.
Abstract
We isolated a Tn5 insertion mutant of Rhodobacter capsulatus which requires biotin for growth. Crossfeeding studies with Escherichia coli bio mutants indicated that the insertion is in the bioB gene. A cosmid that complements the bioB insertion was isolated, and the bioB gene was localized to a 2.85-kilobase EcoRI-PstI fragment.
摘要
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