Isolation of mutants and genes involved in cytochromes c biosynthesis in Rhodobacter capsulatus.
作者信息
Kranz R G
机构信息
Biology Department, Washington University, St. Louis, Missouri 63130.
出版信息
J Bacteriol. 1989 Jan;171(1):456-64. doi: 10.1128/jb.171.1.456-464.1989.
Abstract
Mutants of the photosynthetic bacterium Rhodobacter capsulatus that have combined deficiencies in the cytochrome b/c1 complex and other c-type cytochromes have been isolated. These mutants were unable to grow anaerobically in the light or dark but could grow aerobically. Cosmids with R. capsulatus wild-type DNA that complement the mutants have been used to construct genetic and physical maps of the affected genes. Complementation profiles with Tn5 and mini-Mu insertions in these cosmids and subcloned fragments from them indicated that at least three genes (called helA, helB, and helC) are involved in the defects in cytochromes c biosynthesis. The genes are clustered, and helC is transcribed away from helA and helB. Stable insertion mutants in each gene were constructed. It is postulated that helA, helB, and helC are involved in posttranslational processing during cytochromes c synthesis.
摘要
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本文引用的文献
1
Cytochrome c(2) is not essential for photosynthetic growth of Rhodopseudomonas capsulata.
Proc Natl Acad Sci U S A. 1986 Apr;83(7):2012-6. doi: 10.1073/pnas.83.7.2012.
2
Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti.
Proc Natl Acad Sci U S A. 1980 Dec;77(12):7347-51. doi: 10.1073/pnas.77.12.7347.
3
A quantitative radioimmunological screening method for specific gene products.
Anal Biochem. 1982 Dec;127(2):247-57. doi: 10.1016/0003-2697(82)90169-5.
4
Induction of the photosynthetic membranes of Rhodopseudomonas sphaeroides: biochemical and morphological studies.
J Bacteriol. 1984 Aug;159(2):540-54. doi: 10.1128/jb.159.2.540-554.1984.
5
Plasmid insertion mutagenesis and lac gene fusion with mini-mu bacteriophage transposons.
J Bacteriol. 1984 May;158(2):488-95. doi: 10.1128/jb.158.2.488-495.1984.
6
The use of transposon Tn5 mutagenesis in the rapid generation of correlated physical and genetic maps of DNA segments cloned into multicopy plasmids--a review.
Gene. 1984 Feb;27(2):131-49. doi: 10.1016/0378-1119(84)90135-5.
7
Construction of a broad host range cosmid cloning vector and its use in the genetic analysis of Rhizobium mutants.
Gene. 1982 Jun;18(3):289-96. doi: 10.1016/0378-1119(82)90167-6.
8
Differential accumulation of two apo-iso-cytochromes c in processing mutants of yeast.
J Biol Chem. 1982 Aug 25;257(16):9811-21.
9
Assembly of cytochrome c. Apocytochrome c is bound to specific sites on mitochondria before its conversion to holocytochrome c.
Eur J Biochem. 1981 Dec;121(1):203-12. doi: 10.1111/j.1432-1033.1981.tb06450.x.
10
Genetic-physical mapping of a photosynthetic gene cluster from R. capsulata.
Cell. 1984 Jul;37(3):937-47. doi: 10.1016/0092-8674(84)90428-8.
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