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荚膜红细菌hemA基因的克隆

Cloning of the Rhodobacter capsulatus hemA gene.

作者信息

Biel S W, Wright M S, Biel A J

机构信息

Department of Microbiology, Louisiana State University, Baton Rouge 70803.

出版信息

J Bacteriol. 1988 Sep;170(9):4382-4. doi: 10.1128/jb.170.9.4382-4384.1988.

DOI:10.1128/jb.170.9.4382-4384.1988
PMID:2842318
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC211455/
Abstract

Portions of the Rhodobacter capsulatus hemA gene have been cloned from a hemA::Tn5 insertion strain into the lambda bacteriophage derivative EMBL3. A cosmid containing the wild-type R. capsulatus hemA gene was isolated by complementation of the hemA::Tn5 mutant. The cosmid contains a 1.4-kilobase EcoRI fragment that spans the hemA::Tn5 insertion site. The entire hemA gene is contained in this fragment and the adjacent 0.6-kilobase EcoRI fragment.

摘要

已从一个hemA::Tn5插入菌株中克隆出部分荚膜红细菌hemA基因，并将其插入λ噬菌体衍生物EMBL3中。通过对hemA::Tn5突变体进行互补，分离出了一个含有野生型荚膜红细菌hemA基因的黏粒。该黏粒包含一个1.4千碱基的EcoRI片段，该片段跨越hemA::Tn5插入位点。整个hemA基因包含在这个片段以及相邻的0.6千碱基EcoRI片段中。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/723e/211455/7f52421dfb38/jbacter00187-0602-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/723e/211455/7f52421dfb38/jbacter00187-0602-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/723e/211455/7f52421dfb38/jbacter00187-0602-a.jpg

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本文引用的文献

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Heme biosynthesis in Rhizobium. Identification of a cloned gene coding for delta-aminolevulinic acid synthetase from Rhizobium meliloti.根瘤菌中的血红素生物合成。来自苜蓿根瘤菌的编码δ-氨基乙酰丙酸合成酶的克隆基因的鉴定。
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Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti.用于革兰氏阴性菌的广宿主范围DNA克隆系统：苜蓿根瘤菌基因文库的构建
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