荚膜红细菌hemA基因的克隆
Cloning of the Rhodobacter capsulatus hemA gene.
作者信息
Biel S W, Wright M S, Biel A J
机构信息
Department of Microbiology, Louisiana State University, Baton Rouge 70803.
出版信息
J Bacteriol. 1988 Sep;170(9):4382-4. doi: 10.1128/jb.170.9.4382-4384.1988.
Abstract
Portions of the Rhodobacter capsulatus hemA gene have been cloned from a hemA::Tn5 insertion strain into the lambda bacteriophage derivative EMBL3. A cosmid containing the wild-type R. capsulatus hemA gene was isolated by complementation of the hemA::Tn5 mutant. The cosmid contains a 1.4-kilobase EcoRI fragment that spans the hemA::Tn5 insertion site. The entire hemA gene is contained in this fragment and the adjacent 0.6-kilobase EcoRI fragment.
摘要
已从一个hemA::Tn5插入菌株中克隆出部分荚膜红细菌hemA基因，并将其插入λ噬菌体衍生物EMBL3中。通过对hemA::Tn5突变体进行互补，分离出了一个含有野生型荚膜红细菌hemA基因的黏粒。该黏粒包含一个1.4千碱基的EcoRI片段，该片段跨越hemA::Tn5插入位点。整个hemA基因包含在这个片段以及相邻的0.6千碱基EcoRI片段中。
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