Veterinary Research Institute, Brno, Czech Republic.
FEMS Microbiol Lett. 2011 Aug;321(1):37-42. doi: 10.1111/j.1574-6968.2011.02305.x. Epub 2011 May 31.
In this study, we were interested in the association of attenuated mutants of Salmonella enterica serovar Enteritidis with subpopulations of porcine white blood cells (WBC). The mutants included those with inactivated aroA, phoP, rfaL, rfaG, rfaC and fliC genes and a mutant with five major pathogenicity islands removed (ΔSPI1-5 mutant). Using flow cytometry, we did not observe any difference in the interactions of the wild-type S. Enteritidis, aroA and phoP mutants with WBC. ΔSPI1-5 and fliC mutants had a minor defect in their association with granulocytes and monocytes, but not with T- or B-lymphocytes. All three rfa mutants associated with granulocytes, monocytes and B-lymphocytes more than the wild-type S. Enteritidis did. Electron microscopy confirmed that the association correlated with the intracellular presence of S. Enteritidis and that the Salmonella-containing vacuole in the WBC infected with the rfa mutants, unlike all other strains, did not develop into a spacious phagosome. Intact lipopolysaccharide, but not the type III secretion system encoded by SPI-1, SPI-2 or the flagellar operon, is important for the initial interaction of S. Enteritidis with porcine leukocytes. This information can be used for the design of live Salmonella vaccines preferentially targeting particular cell types including cancer or tumor cells.
在这项研究中，我们对肠炎沙门氏菌血清型 Enteritidis 的衰减突变体与猪白细胞（WBC）亚群的关联感兴趣。这些突变体包括那些具有失活的 aroA、phoP、rfaL、rfaG、rfaC 和 fliC 基因的突变体，以及一个去除了五个主要致病性岛的突变体（ΔSPI1-5 突变体）。使用流式细胞术，我们没有观察到野生型 S. Enteritidis、aroA 和 phoP 突变体与 WBC 之间的相互作用有任何差异。ΔSPI1-5 和 fliC 突变体与粒细胞和单核细胞的结合有轻微缺陷，但与 T 或 B 淋巴细胞没有缺陷。所有三个 rfa 突变体与粒细胞、单核细胞和 B 淋巴细胞的结合都比野生型 S. Enteritidis 更紧密。电子显微镜证实，这种结合与沙门氏菌在 WBC 中的存在有关，与其他所有菌株不同的是，感染 rfa 突变体的 WBC 中的沙门氏菌包含的空泡没有发育成宽敞的吞噬体。完整的脂多糖，而不是由 SPI-1、SPI-2 或鞭毛操纵子编码的 III 型分泌系统，对于 S. Enteritidis 与猪白细胞的初始相互作用很重要。这些信息可用于设计优先针对特定细胞类型（包括癌症或肿瘤细胞）的活沙门氏菌疫苗。
