Laboratory of Microbiology and Biotechnology of Foods, Department of Food Science and Technology, Agricultural University of Athens, Greece.
Food Microbiol. 2011 Aug;28(5):1011-8. doi: 10.1016/j.fm.2011.02.004. Epub 2011 Feb 22.
The effect of acylated homoserine lactones (AHLs) and autoinducer-2 (AI-2) signalling compounds present in the cell-free culture supernatants (CFS), of Pseudomonas aeruginosa, Yersinia enterocolitica-like GTE 112, Serratia proteamaculans 00612, Y. enterocolitica CITY650 and Y. enterocolitica CITY844, on the growth of two Salmonella Enteritidis and two S. Typhimurium strains was assessed though monitoring of changes in conductance of the medium. Detection times (T(det)), area and slope of conductance curves were recorded. Except for P. aeruginosa 108928, which was not found to produce AI-2, all other strains produced both AHLs and AI-2. Thereafter, aliquots (20% in the final volume) of these CFS were transferred into NZ Amine broth inoculated with ca. 10(3) CFU/ml of stationary phase cultures of each Salmonella strain. While the CFS of P. aeruginosa induced a shorter detection time, i.e. acceleration of the metabolic activity, the CFS of the other microorganisms increased the detection time of Salmonella strains compared to control samples (i.e. without CFS). Results indicate that the growth of Salmonella may be affected by the presence of Quorum sensing (QS) signalling compounds and/or other novel signals existing in CFS, produced by other bacterial species and confirm the complexity of bacterial communication.
细胞游离培养上清液（CFS）中酰化高丝氨酸内酯（AHLs）和自动诱导物-2（AI-2）信号化合物对铜绿假单胞菌、类耶尔森氏菌 GTE 112、变形菌属 00612、肠侵袭性大肠杆菌 CITY650 和肠侵袭性大肠杆菌 CITY844 的影响通过监测培养基电导率的变化来评估。记录检测时间（T(det)）、电导率曲线的面积和斜率。除了未发现产生 AI-2 的 108928 株铜绿假单胞菌外，所有其他菌株都产生 AHLs 和 AI-2。此后，将这些 CFS 的等分试样（终体积的 20%）转移到 NZ 胺肉汤中，该肉汤接种了约 10（3）CFU/ml 的每种沙门氏菌菌株的静止期培养物。虽然铜绿假单胞菌的 CFS 诱导检测时间缩短，即代谢活性加速，但与对照样品（即无 CFS）相比，其他微生物的 CFS 增加了沙门氏菌菌株的检测时间。结果表明，存在群体感应（QS）信号化合物和/或其他由其他细菌产生的新型信号 CFS 可能会影响沙门氏菌的生长，并证实了细菌通讯的复杂性。
