三尖杉多糖的抗氧化和保肝活性研究。
Studies on the antioxidant and hepatoprotective activities of polysaccharides from Talinum triangulare.
机构信息
College of Food Science and Technology, Huazhong Agriculture University, No. 1 Shizishan Street of South Lake, Wuhan 430070, China.
出版信息
J Ethnopharmacol. 2011 Jun 22;136(2):316-21. doi: 10.1016/j.jep.2011.04.047. Epub 2011 Apr 29.
UNLABELLED
ETHNOPHAMACOLOGICAL RELEVANCY: The whole plant of Talinum triangulare (Family: Portulacaceae) is used in variety of diseases including hepatic ailments in Africa and Taiwan of China.
AIMS OF THE STUDY
The study was aimed to evaluate the antioxidant and hepatoprotective activity of polysaccharides from T. triangulare (TTP).
MATERIALS AND METHODS
The TTP was extracted using boiling water, and removed protein by Sevag method. 40%, 60% and 80% ethanol precipitating TTP (40%, 60%, 80% TTP) were gained by the successive addition of absolute ethanol. The antioxidant activities of 40%, 60%, 80% and crude TTP were evaluated using three different models in vitro, including reducing power, hydroxyl radicals, superoxide anion. To investigate the hepatoprotective potential, mice were treated with crude polysaccharides (50, 100 and 200mg/kg, p.o.) for 7 days. Liver injures were induced by CCl(4) (0.1% in arachis oil, 10mg/kg, i.v.) 1h after the drug administration on day 7. Mice were sacrificed at 24h after the CCl(4) injection. The levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT) in serum, and glutathione (GSH), superoxide dismutase (SOD), malondialdehyde (MDA) in liver tissues were measured. Histopathological examinations were carried out to supplement the biochemical results.
RESULTS AND CONCLUSIONS
In vitro assays, TTP showed remarkably different degrees of antioxidant activities in dose-dependent manners. The crude TTP demonstrated a relatively strong antioxidant activity, while the 40% TTP showed the strongest antioxidant activity, and the 60% TTP had the weakest antioxidant ability. In vivo assay, pretreatment with TTP had significantly decreased the levels of AST, ALT and MDA against CCl(4) injures, and restored the activities of defense antioxidant substances SOD and GSH towards normalization. These results supported the effect of T. triangulare in fork use with scientific evidence.
目的
研究旨在评估来自 Talinum triangulare（马齿苋科）的多糖的抗氧化和保肝活性。
材料和方法
TTP 采用沸水提取，Sevag 法去除蛋白。通过连续添加绝对乙醇，获得 40%、60%和 80%乙醇沉淀的 TTP（40%、60%、80%TTP）。采用三种不同的体外模型，包括还原力、羟自由基、超氧阴离子，评价 40%、60%、80%和粗 TTP 的抗氧化活性。为了研究其保肝潜力，将小鼠用粗多糖（50、100 和 200mg/kg，po）处理 7 天。在第 7 天给药后 1 小时，用 CCl（4）（0.1%在花生油中，10mg/kg，iv）诱导肝损伤。在 CCl（4）注射后 24 小时处死小鼠。测量血清中天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）的水平，以及肝组织中的谷胱甘肽（GSH）、超氧化物歧化酶（SOD）、丙二醛（MDA）。进行组织病理学检查以补充生化结果。
结果和结论
体外试验中，TTP 表现出明显不同程度的、剂量依赖性的抗氧化活性。粗 TTP 表现出相对较强的抗氧化活性，而 40%TTP 表现出最强的抗氧化活性，60%TTP 具有最弱的抗氧化能力。体内试验中，TTP 预处理显著降低了 CCl（4）损伤对 AST、ALT 和 MDA 的水平，并使防御抗氧化物质 SOD 和 GSH 的活性恢复正常。这些结果为 Talinum triangulare 的民间用途提供了科学依据。
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