Murphy Robert C, Hankin Joseph A, Barkley Robert M, Zemski Berry Karin A
Department of Pharmacology, University of Colorado, Denver, CO, USA.
Biochim Biophys Acta. 2011 Nov;1811(11):970-5. doi: 10.1016/j.bbalip.2011.04.012. Epub 2011 May 5.
Mass spectrometric techniques have been developed to record mass spectra of biomolecules including lipids as they naturally exist within tissues and thereby permit the generation of images displaying the distribution of specific lipids in tissues, organs, and intact animals. These techniques are based on matrix-assisted laser desorption/ionization (MALDI) that requires matrix application onto the tissue surface prior to analysis. One technique of application that has recently shown significant advantages for lipid analysis is sublimation of matrix followed by vapor deposition directly onto the tissue. Explanations for enhanced sensitivity realized by sublimation/deposition related to sample temperature after a laser pulse and matrix crystal size are presented. Specific examples of sublimation/deposition in lipid imaging of various organs including brain, ocular tissue, and kidney are presented.
质谱技术已得到发展，用于记录生物分子（包括脂质）在组织中自然存在时的质谱，从而能够生成显示特定脂质在组织、器官和完整动物体内分布的图像。这些技术基于基质辅助激光解吸/电离（MALDI），在分析之前需要将基质应用于组织表面。一种最近在脂质分析中显示出显著优势的应用技术是基质升华，然后直接将蒸汽沉积到组织上。本文阐述了升华/沉积实现增强灵敏度的原因，这些原因与激光脉冲后的样品温度和基质晶体大小有关。本文还介绍了升华/沉积在包括脑、眼组织和肾脏在内的各种器官脂质成像中的具体实例。
