Holbrook Joseph H, Kemper Gabrielle E, Hummon Amanda B
Ohio State Biochemistry Program, The Ohio State University, Columbus, Ohio 43210, USA.
Department of Chemistry and Biochemistry, The Ohio State University, Columbus, Ohio 43210, USA.
Chem Commun (Camb). 2024 Feb 20;60(16):2137-2151. doi: 10.1039/d3cc05988j.
Mass spectrometry imaging (MSI) has become increasingly utilized in the analysis of biological molecules. MSI grants the ability to spatially map thousands of molecules within one experimental run in a label-free manner. While MSI is considered by most to be a qualitative method, recent advancements in instrumentation, sample preparation, and development of standards has made quantitative MSI (qMSI) more common. In this feature article, we present a tailored review of recent advancements in qMSI of therapeutics and biomolecules such as lipids and peptides/proteins. We also provide detailed experimental considerations for conducting qMSI studies on biological samples, aiming to advance the methodology.
质谱成像（MSI）在生物分子分析中的应用越来越广泛。MSI能够在一次实验中以无标记的方式对数千种分子进行空间定位。虽然大多数人认为MSI是一种定性方法，但仪器、样品制备和标准开发方面的最新进展使定量质谱成像（qMSI）更为常见。在这篇专题文章中，我们对治疗药物和生物分子（如脂质和肽/蛋白质）的qMSI最新进展进行了针对性综述。我们还提供了对生物样品进行qMSI研究的详细实验注意事项，旨在推动该方法的发展。
