Department of Genetics, Washington University Medical School, St Louis, MO 63110, USA.
Dev Cell. 2011 May 17;20(5):725-32. doi: 10.1016/j.devcel.2011.04.013.
We use transposon-based clonal analysis to identify the lineage classes that make the adult zebrafish caudal fin. We identify nine distinct lineage classes, including epidermis, melanocyte/xanthophore, iridophore, intraray glia, lateral line, osteoblast, dermal fibroblast, vascular endothelium, and resident blood. These lineage classes argue for distinct progenitors, or organ founding stem cells (FSCs), for each lineage, which retain fate restriction throughout growth of the fin. Thus, distinct FSCs exist for the four neuroectoderm lineages, and dermal fibroblasts are not progenitors for fin ray osteoblasts; however, artery and vein cells derive from a shared lineage in the fin. Transdifferentiation of cells or lineages in the regeneration blastema is often postulated. However, our studies of single progenitors or FSCs reveal no transfating or transdifferentiation between these lineages in the regenerating fin. This result shows that, the same as in growth, lineages retain fate restriction when passed through the regeneration blastema.
我们使用转座子克隆分析来鉴定构成成年斑马鱼尾鳍的谱系类群。我们确定了九个不同的谱系类群，包括表皮、黑素细胞/黄色素细胞、虹彩细胞、间 ray 神经胶质细胞、侧线、成骨细胞、真皮成纤维细胞、血管内皮细胞和固有血细胞。这些谱系类群表明每个谱系都有独特的祖细胞或器官起始干细胞（FSCs），它们在 fin 的生长过程中保持命运限制。因此，四个神经外胚层谱系存在不同的 FSCs，而真皮成纤维细胞不是 fin 射线成骨细胞的祖细胞；然而，动脉和静脉细胞来源于 fin 中的共享谱系。细胞或谱系在再生芽基中的转分化经常被假设。然而，我们对单个祖细胞或 FSCs 的研究表明，在再生的 fin 中，这些谱系之间没有转分化或转分化。这个结果表明，与生长一样，谱系在通过再生芽基时保持命运限制。
